Polyclonal Antibody against Eps15 (C-term)
产品名称： Polyclonal Antibody against Eps15 (C-term)
英文名称： Polyclonal Antibody against Eps15 (C-term)
产品编号： PRB-124C
产品价格： null
产品产地： USA
品牌商标： COVANCE
更新时间： 2023-08-17T13:40:59
使用范围：
翌圣生物科技（上海）股份有限公司
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640
- 传真 : 021-34615995-188
- 邮箱 : lizizhuan@yeasen.com
| Description |  |
Polyclonal Antibody against Eps15 (C-term) |
| Form | |
Antiserum |
| Host | |
Rabbit |
| Species Reactivity | |
Human, Mouse |
| Specificity | |
The eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form. Several physiological functions have been proposed for eps15. Phosphorylation of EGF and PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and endocytosis. This antibody was generated against a recombinant protein containing the C-termnial sequence of Eps15 (amino acids 539-896). |
| Uses | |
This antibody is effective in immunoblotting, immunoprecipitation and immunoelectron microscopy. |
| Suggested Working Dilution | |
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
|
| Notes | |
*Predicted MW = 145 kD |
| Storage | |
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | |
Cupers P, ter Haar E, Boll W, Kirchhausen T. Parallel dimers and anti-tetramers formed by epidermal growth factor receptor pathways substrate clone 15 (Eps15). J. Biol. Chem. 272(52):33430-33434, 1997. Tebar F, Confalionieri S, Carter RE, Di Fiore PP, Sorkin A. Eps15 is constitutively oligomerized due to homophilic interaction of its coiled-coil region. J. Biol. Chem. 272:15341-15347, 1997. Benmerah A, Gagnon J, Begue B, Megarbane B, Dautry-Varsat A, Cerf-Besussan N. The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2. J. Cell Biol. 131:1831-1838, 1995. Fazioli FL, Minichiello B, Mtoskova W, Wong T, Di Fiore PP. Eps15, a novel tyrosine kinase substrate, exhibits transforming activity. Mol. Cell. Biol. 13:5814-5828, 1993. |
| Warranty/Conditions | |
Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | |
8/21/2006 |