Product Data Sheet

Recovery

1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section o, f the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 ^o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

Growth in Bacteria

Bacterial Resistance(s)  Ampicillin

Growth Temperature  37°C

Growth Strain(s)  DH5alpha

Growth instructions  Stbl3, 37oC

Copy number  High Copy

Gene/Insert

Gene/Insert name  Firefly Luciferase

Alt name  LUC

Insert Size (bp)  1721

Tag / Fusion Protein  V5 (N terminal on backbone)

Cloning Information

Cloning method Restriction Enzyme

5′ cloning site Bam HI (not destroyed)

3′ cloning site Xba I (not destroyed)

5′ sequencing primer PGKforw

>Author sequence   9731bp

caggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatat

gtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtatt

caacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaa

cgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaa

cagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctg

ctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctc

agaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaatt

atgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccg

aaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagc

tgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaa

actattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaa

gttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtg

agcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatcta

cacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgatt

aagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaat

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