Pan anti-acetyllysine rabbit pAb        Cat#PTM-105 


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Pack Size:100 μL                                 Price: ￥2400 
Immunogen: Acetylated BSA              Reactivity: All species expected 

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Product Description: The rabbit-derived pan anti-acetyllysine polyclonal antibody is purified with 

acetyl lysine agarose affinity chromatography. It specifically recognizes proteins with acetyllysine

 residues but not the propionyllysine or butyryllysine residues with structural similarity. 

Except the common applications such as Western blotting, the antibody is capable of 

capturing lysine acetylated peptides from protease-digesting proteins both in prokaryotic 

and eukaryotic cells in systematically proteomic screening. 

Source/Purification: This product is produced by immunizing rabbits with acetylated BSA. 

Antibodies are purified by acetylated lysine agarose affinity chromatography. 

Specificity: Anti-acetyllysine antibody detects proteins post-translationally modified by 

lysine acetylation. The antibody recognizes acetylated lysine in a wide range of sequence contexts. 

The antibody has been shown to have more than 500 times selectivity against acetylated BSA, 

propionylated BSA, and unmodified BSA. 
Recommended Applications: ELISA, WB, IAP 
*WB=Western blotting; IAP=Immuno affinity purification. 
Recommended dilution: WB:1:1000, IP: 1:200. 
IMPORTANT: For Western blotting, incubate membrane with diluted antibody in 5% BSA, 

1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight. 
Use at an assay dependent concentration.Optimal dilutions/concentrations should be determined by 

the end user and maybe assay dependent. Not yet tested in other applications. 


A. Dot blotting analysis on indicated amount of Kac peptide library (lane 1), acetylated BSA (lane 2), 

propionylated BSA (lane 3), butyrylated BSA (lane 4) and unmodified BSA using anti-acetyllysine 

polyclonal antibody. B. Western blotting analysis on 30 ug of crude proteins from HeLa cells using 

anti-acetyllysine polyclonal antibody (1:1000), with (lane2) or without (lane1) competition with 

acetylated BSA.(1:1000) 


Scientific Description: The post-translational ε-amino lysine acetylation of proteins, like phosphorylation 

of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. 

The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation 

of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle 

progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal

 repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the 

mechanistic details of substrate selection and site specificity of these enzymes remain unclear. 

The regulation of protein acetylation status is impaired in the pathologies of cancer and other diseases. 

HDACs have become promising targets for anti-cancer drugs. 

Storage & Stability: Store product at -20℃. Avoid repeated freeze / thaw cycles. Antibody is kept in 

PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt. 

References: 
**This product is intended for research purposes only. The product is not intended to be used for

 therapeutic or diagnostic purposes in humans or animals. 



1. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics, 2009, 8(1),45–52.