MMP9 抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
MMP9 抗体

MMP9 抗体

商家询价

产品名称： MMP9 抗体

英文名称： MMP9 Antibody

产品编号： XY10375-2

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 MMP9 抗体

Applications
In-house applications ELISA, WB, IP, IF, IHC     Application not tested? Please see our Guarantee.
Published applications Dot blotting, IF, IHC, WB Publications 53 citations –view here
Species specificity Human, Mouse, Rat, Zebrafish; other species not tested.
Published Species Human, mouse, rat
Images
Prev12NextView more validation data
  • 10375-2-AP;NIH/3T3 cellsWB results of MMP9 antibody (10375-2-AP, 1:500) with NIH3T3 cells, 92 kDa for pro-MMP9 and 78-82 kDa and 67 kDa for Active MMP9. Data from Dr. Hui-Wen Chiu, Taipei Medical University.
  • 10375-2-AP;Jurkat cellsJurkat cells were subjected to SDS PAGE followed by western blot with 10375-2-AP(MMP9 antibody) at dilution of 1:500
  • 10375-2-AP;Jurkat cellsIP Result of anti-MMP9 (IP:10375-2-AP, 3ug; Detection:10375-2-AP 1:300) with Jurkat cells lysate 4000ug.
  • 10375-2-AP;mouse heart tissueImmunohistochemistry of paraffin-embedded mouse heart tissue slide using 10375-2-AP( MMP9 Antibody) at dilution of 1:200 (under 10x lens).
  • 10375-2-AP;mouse heart tissueImmunohistochemistry of paraffin-embedded mouse heart tissue slide using 10375-2-AP( MMP9 Antibody) at dilution of 1:200 (under 40x lens).
  • 10375-2-AP;HeLa cellsImmunofluorescent analysis of HeLa cells using 10375-2-AP( MMP9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in Jurkat cells, HepG2 cells, mouse heart tissue, NIH/3T3 cells, Raji cells, rat brain tissue, zebrafish tissue
Positive IP detected in Jurkat cells
Positive IHC detected in Mouse heart tissue, human breast cancer tissue, human cervical cancer tissue, human stomach cancer tissue
Positive IF detected in HeLa cells
Recommended dilution WB : 1:200-1:2000
IP : 1:200-1:1000
IHC : 1:100-1:400
IF : 1:20-1:200
 MMP9 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information MMP9 抗体
Immunogen MMP9 fusion protein ag0552 Full name Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Calculated molecular
weight		 707aa,78 kDa Observed molecular 
weight		 67 kDa, 92 kDa
GenBank accession 
number		 BC006093 Gene ID (NCBI) 4318
Gene symbol MMP9 Synonyms 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9
Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades type IV and V collagens. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa and it can be detected a processed form of 68 kDa or 82 kDa and this protein can exsit as a dimer of 180 kDa(PMID:7492685). MMP9 抗体

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