FITC标记的重组金黄色葡萄球菌蛋白A抗体
产品名称： FITC标记的重组金黄色葡萄球菌蛋白A抗体
英文名称： Anti-Protein A/FITC
产品编号： HZ-0362R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-Protein A/FITC Conjugated antibody
FITC标记的重组金黄色葡萄球菌蛋白A抗体
| 英文名称 | Anti-Protein A/FITC |
| 中文名称 | FITC标记的重组金黄色葡萄球菌蛋白A抗体 |
| 别 名 | Immunoglobulin G binding protein A; Staphylococcal protein A; SPA; gG-binding protein A; SPA_STAA8. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Saureus |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 40-60kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant protein A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies. Subcellular Location: Secreted; cell wall. Database links: Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
蛋白A是一种40-60kDa表面蛋白，最初存在于金黄色葡萄球菌（SPA）的细胞壁中。SPA结合许多哺乳动物物种的蛋白质，尤其是IgG，并帮助抑制吞噬性吞噬，并通过这种类型的相互作用作为免疫伪装，因此细菌将破坏调理作用和吞噬作用。已知SPA优先通过与重链的VH3可变区相互作用而与免疫球蛋白的Fc区结合。SPA对人IgG1、IgG2、小鼠IgG2a、IgG2b具有高亲和力，而对人IgM、IgA、IgE以及小鼠IgG3、IgG1具有中等亲和力。SPA通常由大肠杆菌产生，并且实际上与酶、生物素、放射性碘等其他分子偶联，用于免疫学和其他生物学研究。SPA也固定在固体载体上，如琼脂糖珠，用于总IgG纯化或在免疫沉淀研究中鉴定感兴趣的蛋白或蛋白复合物。