Disease:

 Breast ductal carcinoma 

Origin:

 Derived from metastatic pleural effusion 

Species:

Homo sapiens 

Tissue:

Breast 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

This is a hypotriploid human cell line 

Patient:

Female, 54 yrs of age 

Medium:

RPMI1640 + 10% FBS 

Subculture:

1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 calcitonin, androgen receptor, estrogen receptor, progesterone receptor, glucocorticoid receptor, prolactin, 
Isoenzymes:
 AK-1, 1; ES-D, 2; G6PD, B; GLO-I, 1-2; PGM1, 1; PGM3, 1 
DNA Profile (STR):
 Amelogenin: X; CSF1PO: 11,13; D13S317: 12; D16S539: 10; D5S818: 12; D7S820: 11; THO1: 6; TPOX: 11; vWA: 14 
Mutations in T47D Cells

Related cells by Organ:

4T1 

JIMT-1 

MDA-MB-231 

SK-BR-3 

T47D 

MCF-7 


References for P47D cells:

1. Afhuppe, W., Sommer, A., Muller, J., Schwede, W., Fuhrmann, U., and Moller, C. Global gene expression profiling of progesterone receptor modulators in T47D cells provides a new classification system. J Steroid Biochem Mol Biol, 113: 105-115, 2009. 

2. Chen, Y., Huang, K., Chen, K. E., and Walker, A. M. Prolactin and estradiol utilize distinct mechanisms to increase serine-118 phosphorylation and decrease levels of estrogen receptor alpha in T47D breast cancer cells. Breast Cancer Res Treat, 120: 369-377. 

3. Holley, A. K., Kiningham, K. K., Spitz, D. R., Edwards, D. P., Jenkins, J. T., and Moore, M. R. Progestin stimulation of manganese superoxide dismutase and invasive properties in T47D human breast cancer cells. J Steroid Biochem Mol Biol, 117: 23-30, 2009. 

4. Hua, D., Chen, B., Bai, M., Yu, H., Wu, X., and Jin, W. PEA3 activates VEGF transcription in T47D and SKBR3 breast cancer cells. Acta Biochim Biophys Sin (Shanghai), 41: 63-68, 2009. 

5. Sanada, N., Gotoh, Y., Shimazawa, R., Klinge, C. M., and Kizu, R. Repression of activated aryl hydrocarbon receptor-induced transcriptional activation by 5alpha-dihydrotestosterone in human prostate cancer LNCaP and human breast cancer T47D cells. J Pharmacol Sci, 109: 380-387, 2009. 

6. Zhang, Y., Zhao, H., Asztalos, S., Chisamore, M., Sitabkhan, Y., and Tonetti, D. A. Estradiol-induced regression in T47D:A18/PKCalpha tumors requires the estrogen receptor and interaction with the extracellular matrix. Mol Cancer Res, 7: 498-510, 2009.