Synonyms

Interleukin-23

Source

Sf 21 (baculovirus)

Molecular Weight

Apparent molecular mass of 65 kDa, 43-45 kDa and 20 kDa in SDS-PAGE under reducing conditions.

AA Sequence

Human IL-23 p40（Ile23-Ser328）&Human IL-23 p19 (Ala21-Pro189, Accession # AAG37232)

Biological Activity

Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL..

Purity

> 95% by SDS-PAGE.

Endotoxin

<1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.2 μm filtered concentrated sterile solution in 20 mM MES, pH 6.0, 500 mM NaCl and 1 mM EDTA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.