pBabe HcRed
(Plasmid #10677)

PURPOSE

(Empty Backbone)
DEPOSITING LAB

William Hahn
PUBLICATION

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74.
PRICE

$65 (USD)

Add to Cart

Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
pBABE HcRed
(Search Vector Database)
Backbone size (bp)5100
Vector type
Mammalian Expression, Retroviral
Selectable markers
HcRed

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

Gene/Insert name
None

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ sequencing primerpBABE 5
3′ sequencing primerpBABE-3

(Common Sequencing Primers)

RESOURCE INFORMATION

Terms and Licenses
- UBMTA
- Clontech Limited Use Label License

DEPOSITOR COMMENTS

pBabe-puro was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 4.4kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pBabe HcRed was a gift from William Hahn (Addgene plasmid # 10677)
For your References section:

Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784

pBabe HcRed逆病毒载体质粒图谱序列抗性

pBabe HcRed (Plasmid #10677)