Product: Anti-CARD9 pAb
Cat. No.: PC-525 (100 μg)
Synonyms:
Anti-Caspase Recruitment Domain Family 9.
Background:
Apoptosis is related to many diseases and
development. Cell death signals are transduced
by death domain (DD), death effector domain
(DED), and caspase recruitment domain (CARD)
containing molecules. CARD containing proteins
include some caspases, Apaf-1, CARD4, IAPs,
RICK, ARC, RAIDD, BCL-10, and ASC. A novel
CARD-containing protein was recently identified
and designated CARD9, which interacts with the
CARD activation domain of BCL-10. CARD9
associates with BCL-10 and forms a complex
within cells. CARD9 induces apoptosis and
activates NF-
B. CARD9 is an upstream
activator of BCL-10 and NF-
B signaling.
Specificity:
Recognizes CARD9.
Positive Controls:
PC-3 and MDA-MB-361 cell lysates (detects a
band of ~ 59 kDa).
Species Reactivity:
Human. Other species not tested.
Host:
Rabbit
Immunogen:
A synthetic peptide containing 16 amino acids
near the C-terminus of human CARD9.
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 - 1 μg/mL.

Immunocytochemistry: Use at ~ 10 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of CARD9 expression
in human MDA-MB-361 (A) and PC-3 (B) cell lysate
with anti-CARD9 pAb at 2.5 μg/mL.
Figure 2: Immunocytochemistry of CARD9 in K562
cells with anti-CARD9 pAb at 10 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.