pPAN7-1载体质粒
产品名称: pPAN7-1载体质粒
英文名称: pPAN7-1载体质粒
产品编号: BiovectorPPAN71
产品价格: 0
产品产地: Biovector NTCC Inc. USA
品牌商标: Biovector,NTCC, Addgene, ATCC,
更新时间: null
使用范围: null
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Product Data Sheet
Name |
Description |
pAN7-1 |
pAN7-1,in E.coli,200uL,AmpR.Store -20℃ |
Map of the plasmid:
Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
质粒类型: |
真菌表达载体 |
载体大小: |
6756 bp |
载体抗性: |
Ampicillin (氨苄青霉素) |
载体序列:
LOCUS pAN7-1 6756 bp DNA circular SYN 14-NOV-2006
DEFINITION Expression vector (PAN7-1) DNA, 6756bp.
ACCESSION Z32698
VERSION Z32698.1 GI:475166
KEYWORDS hygromycin b phosphotransferase.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
REFERENCE 1
AUTHORS Mullaney,E.J., Hamer,J.E., Roberti,K.A., Yelton,M.M. and
Timberlake,W.E.
TITLE Primary structure of the trpC gene from Aspergillus nidulans
JOURNAL Mol. Gen. Genet. 199 (1), 37-45 (1985)
PUBMED 3158796
REMARK sites .
FEATURES Location/Qualifiers
source 1..6756
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
misc_feature 1..6756
/product="fungal transformation vector pAN7-1"
/standard_name="HPH"
/experiment="experimental evidence, no additional details
recorded"
/citation=[2]
/phenotype="gene product confers resistance to hygromycin
B"
promoter 1..2129
/experiment="experimental evidence, no additional details
recorded"
/citation=[2]
/citation=[3]
/function="promoter region of the A.nidulans gpdA gene"
gene 2130
/gene="gpdA"
misc_feature 2130
/gene="gpdA"
/experiment="experimental evidence, no additional details
recorded"
/citation=[3]
/function="major transcription start site"
intron 2178..2293
/experiment="experimental evidence, no additional details
recorded"
/citation=[3]
/number=1
gene 2302..3321
/gene="HPH"
CDS 2302..3321
/gene="HPH"
/experiment="experimental evidence, no additional details
recorded"
/label="gene-product confers resistance to hygromycin B"
/citation=[2]
/citation=[3]
/codon_start=1
/transl_table=11
/product="hygromycin B phosphotransferase"
/protein_id="CAA83647.1"
/db_xref="GI:475167"
/translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEA
MFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSL
VDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTR
PRAKE"
terminator 3339..4108
/standard_name="terminator region from A.nidulans trpC
gene (containing CDS)"
/experiment="experimental evidence, no additional details
recorded"
/label="this region contains the 3end of the trpC
transcript including 50 codons. two major transcription
ends have been identified"
/citation=[1]
/citation=[2]
polyA_site 3590..3600
misc_difference 3600..3610
/label="introduction of one base at this position generates
an BLGI site with was shown to be present by restriction
digestion"
/citation=[1]
/replace="gccttncaggc"
misc_difference 3809..3817
/label="introduction of one base at this position generates
an ALWNI site which was shown to be present by restriction
digestion"
/citation=[1]
/replace="cagcncctg"
polyA_site 3863..3873
misc_difference 3897..3907
/label="introduction (actual sequence could be deletion as
well) of a base at this position results in the
elimination of an XMNI site which was shown to be absent
by restriction digestion"
/citation=[1]
/replace="gaaatcanttc"
variation 3934..3941
/label="A NCOI site present at this position was removed by
digestion, treatment with klenow polymerase and
religation"
/citation=[2]
/replace="catgcatg"
misc_feature 4105..6756
/product="PUC18"
/label="vector sequences derived from PUC18 from SALI TO
ECORI"
/citation=[2]
ORIGIN
1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
61 cgtttatatc ttgcatcgtc cc