pPAN7-1载体质粒-载体及构建-试剂-生物在线
Biovector Co.,LTD
pPAN7-1载体质粒

pPAN7-1载体质粒

商家询价

产品名称: pPAN7-1载体质粒

英文名称: pPAN7-1载体质粒

产品编号: BiovectorPPAN71

产品价格: 0

产品产地: Biovector NTCC Inc. USA

品牌商标: Biovector,NTCC, Addgene, ATCC,

更新时间: null

使用范围: null

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Product Data Sheet

Name

Description

pAN7-1

pAN7-1,in E.coli,200uL,AmpR.Store -20

Map of the plasmid:

Recovery

1. Obtain an LB agar plate with the appropriate antibiotic.

2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)

3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.

4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.

5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.

6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

质粒类型:

真菌表达载体

载体大小:

6756 bp

载体抗性:

Ampicillin (氨苄青霉素)

载体序列:

LOCUS       pAN7-1                  6756 bp    DNA     circular   SYN 14-NOV-2006
DEFINITION  Expression vector (PAN7-1) DNA, 6756bp.
ACCESSION   Z32698
VERSION     Z32698.1  GI:475166
KEYWORDS    hygromycin b phosphotransferase.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
REFERENCE   1
  AUTHORS   Mullaney,E.J., Hamer,J.E., Roberti,K.A., Yelton,M.M. and
            Timberlake,W.E.
  TITLE     Primary structure of the trpC gene from Aspergillus nidulans
  JOURNAL   Mol. Gen. Genet. 199 (1), 37-45 (1985)
   PUBMED   3158796
  REMARK    sites .
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
     misc_feature    1..6756
                     /product="fungal transformation vector pAN7-1"
                     /standard_name="HPH"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[2]
                     /phenotype="gene product confers resistance to hygromycin
                     B"
     promoter        1..2129
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[2]
                     /citation=[3]
                     /function="promoter region of the A.nidulans gpdA gene"
     gene            2130
                     /gene="gpdA"
     misc_feature    2130
                     /gene="gpdA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[3]
                     /function="major transcription start site"
     intron          2178..2293
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[3]
                     /number=1
     gene            2302..3321
                     /gene="HPH"
     CDS             2302..3321
                     /gene="HPH"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /label="gene-product confers resistance to hygromycin B"
                     /citation=[2]
                     /citation=[3]
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin B phosphotransferase"
                     /protein_id="CAA83647.1"
                     /db_xref="GI:475167"
                     /translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEA
                     MFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSL
                     VDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTR
                     PRAKE"
     terminator      3339..4108
                     /standard_name="terminator region from A.nidulans trpC
                     gene (containing CDS)"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /label="this region contains the 3end of the trpC
                     transcript including 50 codons. two major transcription
                     ends have been identified"
                     /citation=[1]
                     /citation=[2]
     polyA_site      3590..3600
     misc_difference 3600..3610
                     /label="introduction of one base at this position generates
                     an BLGI site with was shown to be present by restriction
                     digestion"
                     /citation=[1]
                     /replace="gccttncaggc"
     misc_difference 3809..3817
                     /label="introduction of one base at this position generates
                     an ALWNI site which was shown to be present by restriction
                     digestion"
                     /citation=[1]
                     /replace="cagcncctg"
     polyA_site      3863..3873
     misc_difference 3897..3907
                     /label="introduction (actual sequence could be deletion as
                     well) of a base at this position results in the
                     elimination of an XMNI site which was shown to be absent
                     by restriction digestion"
                     /citation=[1]
                     /replace="gaaatcanttc"
     variation       3934..3941
                     /label="A NCOI site present at this position was removed by
                     digestion, treatment with klenow polymerase and
                     religation"
                     /citation=[2]
                     /replace="catgcatg"
     misc_feature    4105..6756
                     /product="PUC18"
                     /label="vector sequences derived from PUC18 from SALI TO
                     ECORI"
                     /citation=[2]
ORIGIN      
        1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
       61 cgtttatatc ttgcatcgtc cc