Polyclonal Antibody against Involucrin
产品名称： Polyclonal Antibody against Involucrin
英文名称： Polyclonal Antibody against Involucrin
产品编号： PRB-140C
产品价格： null
产品产地： USA
品牌商标： COVANCE
更新时间： 2023-08-17T13:40:59
使用范围：
翌圣生物科技（上海）股份有限公司
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640
- 传真 : 021-34615995-188
- 邮箱 : lizizhuan@yeasen.com
| Description |  |
Polyclonal Antibody against Involucrin |
| Intended Use | |
**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Form | |
Antiserum |
| Host | |
Rabbit |
| Species Reactivity | |
Mouse |
| Specificity | |
Involucrin serves as a marker for terminal differentiation of stratified squamous epithelia. The involucrin genes have diverged considerably through evolution and as a consequence antibodies specific for the human molecule do not detect the homologous mouse gene product. This polyclonal antibody was generated against an 18 amino acid peptide (ELHLGKQQESHEPDMAED) predicted from the mouse involucrin gene sequence. |
| Uses | |
This antibody is effective in immunoblotting and immunofluorescence (IF). |
| Suggested Working Dilution | |
The optimal working dilution should be determined for each specific assay condition.
|
| Notes | |
*Predicted MW = 56 kDa, however bands ranging from 56-155 kD are detectable depending on nature of source material (refer to Li ER et al paper below). |
| Storage | |
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | |
Merritt AJ, Berika MY, Zhai W, Kirk SE, Ji B, Hardman MJ, Garrod DR. Suprabasal Desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation. Mol Cell Biol 22:5846 - 5858, 2002. Lin AW, Lowe SW. Oncogenic ras activates the ARF-p53 pathway to suppress epithelial cell transformation. PNAS 98:5025-5030, 2001. Djian P, Easley K, Green H. Targeted ablation of the murine involucrin gene. J Cell Biol 151(2):F5-8, 2000. [IF] Li ER, Owens DW, Djian P, Watt FM. Expression of involucrin in normal, hyperproliferative and neoplastic mouse keratinocytes. Exp Dermatol 9(6):431-438, 2000. [Wb] Djian P, Phillips M, Easley K, Huang E, Simon M, Rice RH, Green H. The involucrin genes of the mouse and the rat: study of their shared repeats. Mol Biol Evol 10:1136-1149, 1993. |
| Warranty/Conditions | |
Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | |
3/2/2009 |