he Ambion® mirVana™ miRNA Isolation Kit without Phenol uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)–based method. The method isolates total RNA ranging in size from kilobases down to 10mers. Each kit contains sufficient reagents for up to 40 purifications. Phenol must be purchased separately. 

• Efficient isolation of small RNA–containing total RNA 
• Enrich for small RNA <200 nt to increase sensitivity in downstream analyses 
• Simple 30 minute procedure 
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis 
• Compatible with virtually all cell and tissue types 
• Supplied without phenol 

Efficient Recovery of Small RNA 
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and