Description Histones and other DNA binding proteins play an important role in chromosome organization, gene regulation and posttranslational modification. Core components of histones can be covalently modified through methylation, acetylation, phosphorylation, and sumoylation. These modifications are essential for gene expression, regulation, DNA repair and chromosome condensation. HP-014 is designed to rapidly extract histones and other DNA binding proteins from cultured cells and cells isolated from animal tissues. Unlike many other commercial histone extraction kits that employ acid or high salt extraction methods, HP-014 extracts histone proteins through a completely different mechanism. This kit is equally efficient in extracting histones and other DNA binding proteins that can be used for internal controls for histone modification analysis. In comparison, acid extraction protocol selectively extracts basic proteins and a good internal control is usually not possible. HP-014 can extract histones from 0.5 to 5 million cells in less than 10 min with high protein yield (1-2.5 mg/ml) making it the fastest and most robust histone extraction kit available in the market.
Application The kit is designed to rapidly and efficiently extract histones and other DNA binding proteins (regardless of binding affinity) for applications such as SDS-PAGE, immunoblottings, ELISA, IP and protein localization and modification studies.
Buffer Formulation: Proprietary
Kit Components 1. 25 ml buffer A 2. 25 ml buffer B 3. 50 protein extraction cartridges 4. 50 collection tubes with cap
Storage: Store the kit at room temperature.
Important Product Information The use of protease inhibitors is optional for this kit. If downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to the extract is recommended. For determination of protein concentration, BCA kit (Pierce) is recommended. To study protein phosphorylation,
phosphatase inhibitors (such as PhosStop from Roche) should be added to buffer A prior to use.