Monoclonal Antibody against Mannosidase II
产品名称： Monoclonal Antibody against Mannosidase II
英文名称： Monoclonal Antibody against Mannosidase II
产品编号： MMS-110R
产品价格： null
产品产地： USA
品牌商标： COVANCE
更新时间： 2023-08-17T13:40:59
使用范围：
翌圣生物科技（上海）股份有限公司
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640
- 传真 : 021-34615995-188
- 邮箱 : lizizhuan@yeasen.com
| Description |  |
Monoclonal Antibody against Mannosidase II |
| Intended Use | |
**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Clone | |
53FC3 |
| Form | |
Ascites |
| Host | |
Mouse |
| Species Reactivity | |
Rodent |
| IsoType | |
IgG2b |
| [Ab] | |
3-5 mg/mL |
| Specificity | |
The 53FC3 antibody reacts with Mannosidase II, a 135kD protein located on the luminal side of the Golgi membranes. This antibody is useful as an immunocytochemical marker for the Golgi apparatus as well as for studying the fate of the Golgi complex in dividing cells. |
| Uses | |
This antibody is effective in immunofluorescence (IF) and is known not to work in immunoblotting. |
| Suggested Working Dilution | |
The optimal working dilution should be determined for each specific assay condition.
|
| Notes | |
*Detection may be tissue specific (i.e. the antibody detects mannosidase II in BHK cells but not CHO cells). |
| Storage | |
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | |
Zuber C, Spiro MJ, Guhl B, Spiro RG, Roth J. Golgi apparatus immunolocalization of endomannosidase suggests post-endoplasmic reticulum glucose trimming: implications for quality control. Mol Biol Cell 11(12):4227-4240, 2000. Baron MD, Garoff H. Mannosidase II and the 135-kD Golgi-specific antigen recognized by monoclonal antibody 53FC3 are the same dimeric protein. J Biol Chem 265:19928-19931, 1990. Burke B, Warren G. Microinjection of mRNA coding for an anti-Golgi antibody inhibits intracellular transport of a viral membrane protein. Cell 36:847-856, 1984. Burke B, Griffiths G, Reggio H, Louvard D, Warren G. A monoclonal antibody against a 135-K Golgi membrane protein. EMBO J 1:1621-1628, 1982. |
| Warranty/Conditions | |
Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | |
8/7/2007 |