Product: Anti-Bcl-B pAb
Cat. No.: PC-519 (100 μg)
Synonyms:
Anti-Bcl-2L10, anti-Boo, anti-Diva, anti-Nrh.
Background:
Members in the Bcl-2 family are critical
regulators of apoptosis by either inhibiting or
promoting cell death. Bcl-B is a recently
discovered anti-apoptotic member of the Bcl-2.
Unlike the mouse homolog (also known as
Diva/Boo), which is predominantly expressed in
ovary and testis, the human Bcl-B protein is
widely expressed. Also, the human Bcl-B
functions by binding to and suppressing the
apoptotic activity of Bax, whereas the mouse
homolog binds Bak and also interacts with the
apoptosis protein Apaf-1. Despite its predicted
molecular weight, Bcl-B is often at higher
molecular weights, presumably due to posttranslational
modifications.
Specificity:
Recognizes Bcl-B.
Positive Control:
Jurkat cell lysate
Species Reactivity:
Human and mouse. Other species not tested.
Host:
Rabbit
Immunogen:
A 16 amino acid peptide from near the aminoterminus
of human Bcl-B (GenBank Accession
No. NP_065129).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 1-2 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Western blot analysis of Bcl-B in Jurkat lysate
with anti-Bcl-B pAb at 1 μg/mL in the (A) absence and
(B) presence of blocking peptide.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product