甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)重组蛋白

YB895Mu022
Recombinant Mannose Associated Serine Protease 1 (MASP1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revisedd in Jul, 2013)
甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)重组蛋白[ PROPERTIES ]
Residues: His20~Ala447 (Accession # P48740),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.2
甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)重组蛋白pplications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)重组蛋白MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- H TVELNNMFGQ IQSPGYPDSY
PSDSEVTWNI TVPDGFRIKL YFMHFNLESS YLCEYDYVKV ETEDQVLATF CGRETTDTEQ
TPGQEVVLSP GSFMSITFRS DFSNEERFTG FDAHYMAVDV DECKEREDEE LSCDHYCHNY
IGGYYCSCRF GYILHTDNRT CRVECSDNLF TQRTGVITSP DFPNPYPKSS ECLYTIELEE
GFMVNLQFED IFDIEDHPEV PCPYDYIKIK VGPKVLGPFC GEKAPEPIST QSHSVLILFH
SD NS GE N R GW RL S Y RAA G NE CP E LQP P V H G KI E P SQ AK Y F FK D QV LV S C D
TGYKVLKDNV EMDTFQIECL KDGTWSNKIP TCKIVDCRAP GELEHGLITF STRNNLTTYK
SEIKYSCQEP YYKMLNNNTG IYTCSAQGVW MNKVLGRSLP TCLPVCGLPK FSRKLMA