目录号 
            
规格 
            
价格 
            
说明书下载 
        
        

            
C418A
            
2μg
            
400元
            

        
        

            
C418B
            
10μg
            
1800元
        
        

            
C418C
            
50μg
            
4500元
        
    

 
TNFRSF14, also known as Herpesvirus entry  mediator (HVEM), TR2 (TNF receptor like molecule) and ATAR (another TRAF  associated receptor), is a type I membrane protein belonging to the  TNF/NGF receptor superfamily. The extracellular domain of HVEM contains  several cysteine rich repeats characteristic of TNF receptor superfamily  members. The short cytoplasmic region lacks a death domain present in  some TNF receptor family members, but contains a TRAF (TNF receptor  associated factor) interaction domain. The extracellular domain of HVEM  has been shown to interact directly with the herpes simplex virus(HSV)  envelope glycoprotein D which plays an important role in HSV  pathogenesis. Two TNF superfamily ligands, including the secreted TNFβ  (lymphotoxin α) and the membrane protein LIGHT competes with HSV  glycoprotein D for HVEM.The cytoplasmic region of HVEM was found to bind  to several TRAF family members, which may mediate the signal  transduction pathways that activate the immune response


·Description

Recombinant Human TNFRSF14 produced by  transiently transfected human cell is the extracellular  domain(Accession#Q92956) (Leu39-Val202) of human TNFRSF14 fused with a  polyhistidine tag at the C-terminus.


·Quality Control
Biological activity:The ED50 of  rHuTNFRSF14 is typically 2.5-10μg/mL in the presence of 1 ng/mL of  rhTNFß, as measured by its ability to inhibit TNFß mediated cytotoxicity  using L929 mouse fibrosarcoma cells.
Purity: Greater than 90.0% as determined by: 

(a) Analysis by RP-HPLC. 

(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. 

Amino-Acid Sequence: Predicted N terminal amino acid is Leu39.
Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHuTNFRSF14.
 
·Formulation

 Sterile and Lyophilized from a 0.2 μm filtered solution in PBS.
·Reconstitution

It is recommended to reconstitute the  lyophilized rHuTNFRSF14 in sterile water not less than 100μg/ml, which  can then be further diluted to other aqueous solutions.
·Storage 

Lyophilized rHuTNFRSF14 although stable at  room temperature for 3 weeks, should be stored desiccated below -18°C.  Upon reconstitution rHuTNFRSF14 should be stored at 4°C between 2-7 days  and aliquots of reconstituted samples containing 0.1% BSA are stable up  to 3 months below -18°C for future use.