pMKO.1 puro
(Plasmid #8452)

PURPOSE

(Empty Backbone)
DEPOSITING LAB

Bob Weinberg
PUBLICATION

Stewart et al RNA 2003 Apr;9(4):493-501.
PRICE

$65 (USD)

Add to Cart

Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
pMSCV
(Search Vector Database)
Backbone size (bp)6700
Vector type
Mammalian Expression, Retroviral, RNAi
Selectable markers
Puromycin

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

Gene/Insert name
None

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ cloning siteAgeI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primerpLXSN 5
3′ sequencing primerpBABE-3

(Common Sequencing Primers)

RESOURCE INFORMATION

Supplemental documents
- Weinberg-viral
Terms and Licenses
- UBMTA

DEPOSITOR COMMENTS

Empty MuLV vector for siRNA expression, replaces Retrohair. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pMKO.1 puro was a gift from Bob Weinberg (Addgene plasmid # 8452)
For your References section:

Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD. RNA 2003 Apr;9(4):493-501.10.1261/rna.2192803 PubMed 12649500

pMKO.1 puro逆病毒载体质粒图谱序列抗性

pMKO.1 puro (Plasmid #8452)