pMSCVpuroATT
(Plasmid #47539)

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PURPOSE

(Empty Backbone) Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
DEPOSITING LAB

David Weinstock
PUBLICATION

Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.1371/journal.pone.0049201. Epub 2012 Nov 7.
PRICE

$65 (USD)

Add to Cart

Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
pMSCVpuro
(Search Vector Database)
Backbone manufacturer
Clontech
Backbone size (bp)6300
Modifications to backbone
Inserted Reading Frame Cassette A into the retroviral expression vector pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
Vector type
Mammalian Expression, Retroviral
Selectable markers
Puromycin

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin and Chloramphenicol
Growth Temperature
37°C
Growth Strain(s)
ccdB Survival
Copy number
Unknown

SEQUENCE INFORMATION

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

Cloning methodGateway Cloning
5′ sequencing primerpLXSN-5
3′ sequencing primerMSCV-rev

(Common Sequencing Primers)

RESOURCE INFORMATION

Terms and Licenses
- UBMTA

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pMSCVpuroATT was a gift from David Weinstock (Addgene plasmid # 47539)
For your References section:

Next-generation cDNA screening for oncogene and resistance phenotypes. Shindoh N, Yoda A, Yoda Y, Sullivan TJ, Weigert O, Lane AA, Kopp N, Bird L, Rodig SJ, Fox EA, Weinstock DM. PLoS One. 2012;7(11):e49201. doi: 10.1371/journal.pone.0049201. Epub 2012 Nov 7. 10.1371/journal.pone.0049201 PubMed 23145123

pMSCVpuroATT逆病毒载体质粒图谱序列抗性